For production mode, we recommend using the T7 approach for generating probes. See protocol in Beliveau, et al. 2017 (Methods Mol Biol 2017 1663:231-252. PMID 28924672 and references within). Probe Synthesis Protocols Bulk_emPCR_setup_for_Oligopaints_1_3.pdf Bulk_Emulsion_Breaking_Protocol_2_3.pdf Bulk_purification_of_labeled_ssDNA_3_3.pdf lamda_exonuclease_probe_generation_protocol.pdf Oligopaints_Full_Synthesis_Protocol.pdf FISH Protocols Interphase_FISH_protocol.pdf Simultaneous_RNA_DNA_FISH_protocol.pdf Slide_preparation_for_interphase_FISH.pdf